The march began at the Absan Al-Jadeda village east of Khan Younis toward to the zone, with protesters waving Palestinian flags and banners denouncing Israel's ban on access to the area that it considers a live combat zone.
Protesters said Israeli forces opened fire as they arrived at the area, where they placed Palestinian flags. No injuries were reported.
Imad Asfour, the Khan Younis campaign coordinator, condemned Israel's opening of fire and said the area was bulldozed daily.
During a speech at the rally, Adnan Al-Faq'awi said the Popular Campaign will continue to host marches and peaceful activities against the buffer zone in the eastern and northern part of Gaza, despite the threat of Israeli fire. He called for support for farmers affected by the no-go zone.
Al-Faq'awi further called for Hamas and Fatah to reconcile to defend Palestinian land from further land confiscation.