In Bilin, northwest of Ramallah, local activists said Israeli soldiers fired dozens of tear gas canisters at the protesters as they reached a barrier the Israeli army was building on village land to separate it from nearby Jewish settlements.
Many suffered from tear gas inhalation and required treatment, said the activists.
The Bilin protests, which has been going on for six years, was successful in removing the barrier further back and salvage parts of their agricultural land.
In Al-Masra, near Bethlehem, three young Palestinians were hurt when soldiers attacked the weekly protest with clubs, said residents.
The soldiers attacked the protesters as they walked toward the barrier the army was building on their land to separate it from nearby settlements.
Other similar protests took place in Nilin and Nabi Saleh, in the Ramallah area.